A problem of automated image processing of transmission electron microscopy and its application value is considered in this paper. An automated algorithm for estimating the interplanar distances of the crystal structure of a substance from transmission electron microscopy images is proposed. The software implementation of the algorithm was developed and tested on several raster images, and the evaluation results were compared with the results obtained using the specialized software named Gatan Microscopy Suite v.1.8. The high degree of coincidence of the results showed the viability of the proposed approach and the prospects of its further development in the area of transmission electron microscopy images processing. Copyright © 2019 for this paper by its authors.